Insertional activation of a promoterless thymidine kinase gene.

Restriction Enzyme Analysis of Thymidine Kinase Gene in Four Iranian Isolates of Herpes Simplex Virus Type 1

Insertional gene activation by lentiviral and gammaretroviral vectors.

Gammaretroviral and lentiviral vectors are promising tools for gene therapy, but they can be oncogenic. The development of safer vectors depends on a quantitative assay for insertional mutagenesis. Here we report a rapid, inexpensive, and reproducible assay which uses a murine cell line to measure the frequency of interleukin-3 (IL-3)-independent mutants. Lentiviral and gammaretroviral vectors ...

Post - lranscripdonal regulation of the ctikken thymidine kinase gene

In attempting to understand the molecular basis of the control of chicken thymidine kinase (cTK) gene expression, we have examined the steady state cTK RNA content, and the patterns of DNA methylation, chromatin structure and endogenous nuclear runoff transcription of this gene in dividing and nondividing cells. Our results reveal that the steady state level of cTK poly A RNA is correlated with...

Activation of cam promoterless gene by ISR10 transposition in an Escherichia coli population under stress conditions

The occurrence of promoter-generating mutations allowing the transcription of a promoterless gene has been studied in a system based on the broad-host range promoterprobe plasmid pAF300, harbouring the cam promoterless genes, in an Escherichia coli population sensitive to chloramphenicol. Under selective stressful conditions, Camr colonies accumulated in an E. coli Cams culture. The number of C...

Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA.

Transformation of human cells from a thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.75)-negative to a thymidine kinase-positive phenotype has been achieved by using purified DNA from herpes simplex virus type 2. The specific activity of the DNA was in the range 0.5 to 2.0 transformants per microng and the efficiency of gene transfer was up to 1 transformant per 10(5) recipient ...